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Aitc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aitc  (Tokyo Chemical Industry)
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Effects of RCS scavengers on ITC‐induced stomatal closure. Arabidopsis rosette leaves were treated with 50 μM <t>allyl</t> <t>isothiocyanate</t> <t>(AITC),</t> sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (C), for 30 min. Each bar is presented as the mean of three replications (60 stomata per bar) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test). The images show Arabidopsis guard cells treated with ITCs in the absence and presence of carnosine (B) or pyridoxamine (D). Scale bar: 5 μm.
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mustard oil (allyl isothiocyanate [aitc)]  (Burlington Industries)
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Effects of RCS scavengers on ITC‐induced stomatal closure. Arabidopsis rosette leaves were treated with 50 μM <t>allyl</t> <t>isothiocyanate</t> <t>(AITC),</t> sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (C), for 30 min. Each bar is presented as the mean of three replications (60 stomata per bar) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test). The images show Arabidopsis guard cells treated with ITCs in the absence and presence of carnosine (B) or pyridoxamine (D). Scale bar: 5 μm.
Mustard Oil (Allyl Isothiocyanate [Aitc)], supplied by Burlington Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aitc  (FUJIFILM)
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Effects of RCS scavengers on ITC‐induced stomatal closure. Arabidopsis rosette leaves were treated with 50 μM <t>allyl</t> <t>isothiocyanate</t> <t>(AITC),</t> sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (C), for 30 min. Each bar is presented as the mean of three replications (60 stomata per bar) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test). The images show Arabidopsis guard cells treated with ITCs in the absence and presence of carnosine (B) or pyridoxamine (D). Scale bar: 5 μm.
Aitc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aitc  (Nacalai)
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Effects of RCS scavengers on ITC‐induced stomatal closure. Arabidopsis rosette leaves were treated with 50 μM <t>allyl</t> <t>isothiocyanate</t> <t>(AITC),</t> sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (C), for 30 min. Each bar is presented as the mean of three replications (60 stomata per bar) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test). The images show Arabidopsis guard cells treated with ITCs in the absence and presence of carnosine (B) or pyridoxamine (D). Scale bar: 5 μm.
Aitc, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aitc  (Kuang Lung Shing)
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Kuang Lung Shing aitc
Effects of RCS scavengers on ITC‐induced stomatal closure. Arabidopsis rosette leaves were treated with 50 μM <t>allyl</t> <t>isothiocyanate</t> <t>(AITC),</t> sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (C), for 30 min. Each bar is presented as the mean of three replications (60 stomata per bar) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test). The images show Arabidopsis guard cells treated with ITCs in the absence and presence of carnosine (B) or pyridoxamine (D). Scale bar: 5 μm.
Aitc, supplied by Kuang Lung Shing, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of RCS scavengers on ITC‐induced stomatal closure. Arabidopsis rosette leaves were treated with 50 μM allyl isothiocyanate (AITC), sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (C), for 30 min. Each bar is presented as the mean of three replications (60 stomata per bar) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test). The images show Arabidopsis guard cells treated with ITCs in the absence and presence of carnosine (B) or pyridoxamine (D). Scale bar: 5 μm.

Journal: Physiologia Plantarum

Article Title: Reactive Carbonyl Species Mediate Isothiocyanate Signaling Pathway in Arabidopsis thaliana Guard Cells

doi: 10.1111/ppl.70775

Figure Lengend Snippet: Effects of RCS scavengers on ITC‐induced stomatal closure. Arabidopsis rosette leaves were treated with 50 μM allyl isothiocyanate (AITC), sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (C), for 30 min. Each bar is presented as the mean of three replications (60 stomata per bar) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test). The images show Arabidopsis guard cells treated with ITCs in the absence and presence of carnosine (B) or pyridoxamine (D). Scale bar: 5 μm.

Article Snippet: Then, 0.1% dimethyl sulfoxide or 50 μM AITC (Tokyo Chemical Industry [TCI], purity > 95.0%, specific gravity = 1.02), SFN (Sigma Aldrich), BITC (TCI, purity > 97.0%, specific gravity = 1.21), or PEITC (TCI, purity > 97.0%, specific gravity = 1.11) (all were dissolved in dimethyl sulfoxide) or acrolein (TCI, purity > 97.0%, specific gravity = 0.85) or HNE (Enzo Life Science) or crotonaldehyde (TCI, purity > 98.0%, specific gravity = 0.85) or ( E )‐2‐pentenal (TCI, purity > 95.0%, specific gravity = 0.85) or propionaldehyde (TCI, purity > 98.0%, specific gravity = 0.81) or butyraldehyde (TCI, purity > 98.0%, specific gravity = 0.81) or n ‐pentanal (TCI, purity > 95.0%, specific gravity = 0.82) was added to the solution for another 2 h incubation under light condition.

Techniques:

Effects of RCS scavengers on ITC‐induced elevation of ROS levels in guard cells. Epidermal tissues were incubated with 2′,7′‐dichlorodihydrofluorescein diacetate for 30 min and then were treated with 50 μM allyl isothiocyanate (AITC), sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) in the absence or presence of carnosine (A) or pyridoxamine (B). Dimethyl sulfoxide (0.1%, control) does not affect ROS levels. Samples were pretreated with RCS scavengers for 30 min before ITCs application. The vertical axis shows the percentage of ROS levels. Each bar shows the average data of 60 guard cells ( n = 3) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test).

Journal: Physiologia Plantarum

Article Title: Reactive Carbonyl Species Mediate Isothiocyanate Signaling Pathway in Arabidopsis thaliana Guard Cells

doi: 10.1111/ppl.70775

Figure Lengend Snippet: Effects of RCS scavengers on ITC‐induced elevation of ROS levels in guard cells. Epidermal tissues were incubated with 2′,7′‐dichlorodihydrofluorescein diacetate for 30 min and then were treated with 50 μM allyl isothiocyanate (AITC), sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) in the absence or presence of carnosine (A) or pyridoxamine (B). Dimethyl sulfoxide (0.1%, control) does not affect ROS levels. Samples were pretreated with RCS scavengers for 30 min before ITCs application. The vertical axis shows the percentage of ROS levels. Each bar shows the average data of 60 guard cells ( n = 3) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test).

Article Snippet: Then, 0.1% dimethyl sulfoxide or 50 μM AITC (Tokyo Chemical Industry [TCI], purity > 95.0%, specific gravity = 1.02), SFN (Sigma Aldrich), BITC (TCI, purity > 97.0%, specific gravity = 1.21), or PEITC (TCI, purity > 97.0%, specific gravity = 1.11) (all were dissolved in dimethyl sulfoxide) or acrolein (TCI, purity > 97.0%, specific gravity = 0.85) or HNE (Enzo Life Science) or crotonaldehyde (TCI, purity > 98.0%, specific gravity = 0.85) or ( E )‐2‐pentenal (TCI, purity > 95.0%, specific gravity = 0.85) or propionaldehyde (TCI, purity > 98.0%, specific gravity = 0.81) or butyraldehyde (TCI, purity > 98.0%, specific gravity = 0.81) or n ‐pentanal (TCI, purity > 95.0%, specific gravity = 0.82) was added to the solution for another 2 h incubation under light condition.

Techniques: Incubation, Control

Effects of RCS scavengers on ITC‐induced GSH content in guard cells. Epidermal tissues were treated with 50 μM allyl isothiocyanate (AITC), sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) in the presence of monochlorobimane for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (B), for 30 min. Dimethyl sulfoxide (0.1%, control) as a solvent does not affect GSH content in guard cells. The vertical axis shows the percentage of GSH content. Each bar shows the average data of 60 guard cells ( n = 3) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test).

Journal: Physiologia Plantarum

Article Title: Reactive Carbonyl Species Mediate Isothiocyanate Signaling Pathway in Arabidopsis thaliana Guard Cells

doi: 10.1111/ppl.70775

Figure Lengend Snippet: Effects of RCS scavengers on ITC‐induced GSH content in guard cells. Epidermal tissues were treated with 50 μM allyl isothiocyanate (AITC), sulforaphane (SFN), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) in the presence of monochlorobimane for 2 h after pretreated with RCS scavengers, carnosine (A) and pyridoxamine (B), for 30 min. Dimethyl sulfoxide (0.1%, control) as a solvent does not affect GSH content in guard cells. The vertical axis shows the percentage of GSH content. Each bar shows the average data of 60 guard cells ( n = 3) ± standard error of the mean. Different letters indicate statistical significance ( p < 0.05, with Tukey's test).

Article Snippet: Then, 0.1% dimethyl sulfoxide or 50 μM AITC (Tokyo Chemical Industry [TCI], purity > 95.0%, specific gravity = 1.02), SFN (Sigma Aldrich), BITC (TCI, purity > 97.0%, specific gravity = 1.21), or PEITC (TCI, purity > 97.0%, specific gravity = 1.11) (all were dissolved in dimethyl sulfoxide) or acrolein (TCI, purity > 97.0%, specific gravity = 0.85) or HNE (Enzo Life Science) or crotonaldehyde (TCI, purity > 98.0%, specific gravity = 0.85) or ( E )‐2‐pentenal (TCI, purity > 95.0%, specific gravity = 0.85) or propionaldehyde (TCI, purity > 98.0%, specific gravity = 0.81) or butyraldehyde (TCI, purity > 98.0%, specific gravity = 0.81) or n ‐pentanal (TCI, purity > 95.0%, specific gravity = 0.82) was added to the solution for another 2 h incubation under light condition.

Techniques: Control, Solvent